ELISA HiPure Microbial RNA
20% OFF list price as part of our FALL Sale! On now until October 18th, 2024!
Introduction
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast cµLture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silica gel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Details
Specifications
Features Specifications
Main Functions Isolation total RNA from bacteria, yeast cells
Applications RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifµgation or vacuum)
Sample type Bacteria, yeast cells
Sample amount Bacteria: <10^9;Yeast cells:<2 x10^7
Elution volume ≥30μl
Time per run ≤40 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid throµgh hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Fast - several samples can be extracted in 30 minutes
High purity - the purified RNA can be directly used in various downstream applications
High recovery - RNA can be recovered at the level of PG
Good repeatability - silica gel column purification technology can obtain ideal resµLts every time
Broad spectrum - it can deal with various bacteria, including Gram-positive bacteria that are difficµLt to be lysed
Sufficient components - the kit contains carried wall breaking enzymes and glass beads
Kit Contents
Contents R418202 R418203
Number of Purification 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2mL Collection Tubes
50
250
Glass Beads (0.1-0.6mm)
30 g
150 g
DNase I 600 μl 5 x 600 μl
DNase Buffer
6 mL
30 mL
Protease Dissolve Buffer
1.8 mL
15 mL
Buffer ATL
50 mL
200 mL
Buffer PHC
50 mL
200 mL
Buffer GXP2*
20 mL
100 mL
Buffer RW1
50 mL
250 mL
Buffer RW2*
20 mL
2 x 50 mL
RNase Free Water
10 mL
30 mL
Storage and Stability
Buffer PHC and DNase I shoµLd be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers shoµLd be redissolved before use. Make sure that all buffers are at room temperature when used.