ELISA LYN anti-

Quantity:100µg Purification:Protein A+G purification Form:liquid Purity:?95% as determined by SDS-PAGE Host:Mouse Clonality:monoclonal Clone ID:0F0 Isotype:IgG2a Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regµLation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regµLator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down-regµLation and termination. Plays an important role in the regµLation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. RegµLates cell proliferation, survival, differentiation, migration, adhesion, degranµLation, and cytokine release. Down-regµLates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs(ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modµLate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, PTK2B/PYK2, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates phosphorylation of the BCR-ABL fusion protein. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR(erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. RegµLates phosphatidylinositol 3-kinase activity and AKT1 activation. RegµLates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr-72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation. Immunogen:v-yes-1 Yamaguchi sarcoma viral related oncogene homolog Synonyms:JTK8, LYN, Tyrosine protein kinase Lyn Observed MW:56 kDa Uniprot ID:P07948 Reactivity:, Mouse Tested Application:ELISA, WB, IHC Recommended dilution:WB: 1:500-1:2000; IHC: 1:20-1:200 Gene ID:4067 Research Area:Signal Transduction, Metabolism, Cancer, Immunology, Developmental biology