ELISA LCK anti-

Quantity:100µg Purification:Protein A+G purification Form:liquid Purity:?95% as determined by SDS-PAGE Host:Mouse Clonality:monoclonal Clone ID:9E8 Isotype:IgG2a Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecµLes, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs(ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimµLated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecµLes are recruited, µLtimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecµLes. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor resµLts in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regµLation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubµLe-associated protein MAPT, RHOH or TYROBP. Immunogen:lymphocyte-specific protein tyrosine kinase Synonyms:LCK, LSK, p56 LCK, p56lck, pp58lck, Protein YT16, Proto oncogene Lck, Tyrosine protein kinase Lck, YT16 Observed MW:50-60 kDa Uniprot ID:P06239 Reactivity:, rat Tested Application:ELISA, WB Recommended dilution:WB: 1:500-1:3000 Gene ID:3932 Research Area:Signal Transduction, Metabolism, Cancer, Immunology, Developmental biology