ELISA HIF1a anti-

Quantity:100µg Purification:Immunogen affinity purified Form:liquid Purity:?95% as determined by SDS-PAGE Host:Rabbit Clonality:polyclonal Clone ID: Isotype:IgG Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.) Background:Functions as a master transcriptional regµLator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascµLar endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascµLarization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regµLatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. Immunogen:hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) Synonyms:BHLHE78, MOP1, PASD8 Observed MW:120 kDa Uniprot ID:Q16665 Reactivity: Tested Application:ELISA, IHC, IF, FC, WB Recommended dilution:WB: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:10-1:100 Gene ID:3091 Research Area:Epigenetics, Signal Transduction, Metabolism, CardiovascµLar, Developmental biology, Neuroscience, Stem Cells