DNeasy Plant Pro Kit - 50 preps
The DNeasy Plant Pro Kit is a reliable solution for rapid and efficient extraction of high-purity DNA from plant tissues. Designed for 50 preps, this kit is ideal for researchers requiring consistent results in plant genomics, molecular biology, and genetic analysis.
Featuring optimized protocols and advanced reagents, the kit efficiently isolates DNA from a wide range of plant species, including difficult samples with high levels of contaminants. Perfect for downstream applications such as PCR, qPCR, and next-generation sequencing.
Order : Info@gentaur.com
Featuring optimized protocols and advanced reagents, the kit efficiently isolates DNA from a wide range of plant species, including difficult samples with high levels of contaminants. Perfect for downstream applications such as PCR, qPCR, and next-generation sequencing.
Order : Info@gentaur.com
This content will be shared across all product pages.
DNeasy Plant Pro Kit: Protocol for DNA Extraction from Plant Samples
The DNeasy Plant Pro Kit is an essential tool for researchers and scientists working with plant DNA extraction. This kit is designed to quickly and efficiently extract high-quality genomic DNA from a variety of plant tissues, making it ideal for plant genomics, gene expression studies, and molecular biology research.
DNeasy Plant Pro Kit Protocol
Materials Needed :
- DNeasy Plant Pro Kit
- Plant tissue samples (up to 100 mg)
- Ethanol (96-100%)
- PBS or 1X TE buffer
- Optional: RNase A (if RNA contamination is a concern)
Sample Preparation :
- Collect fresh or frozen plant material (up to 100 mg).
- If working with tough plant tissues like wood, seeds, or roots, grind the sample in a liquid nitrogen environment using a pestle and mortar or a bead mill.
Lysis :
- Add the ground tissue to the lysis buffer provided in the DNeasy Plant Pro Kit. The proprietary lysis buffer effectively breaks down plant cell walls and releases the genomic DNA.
- Vortex the mixture thoroughly to ensure complete lysis. Incubate for 10-15 minutes at room temperature (RT).
Binding DNA :
- Add the lysate to the DNeasy silica membrane column. The DNA will bind to the membrane, and other cellular components will be removed through subsequent washing steps.
- Centrifuge at 6,000 x g for 1 minute to bind the DNA.
Washing :
- Add wash buffer to the column and centrifuge to remove any contaminants or residual reagents. Repeat the wash step using a fresh wash buffer until the column is free from any impurities.
DNA Elution :
- Elute the DNA by adding elution buffer to the column and incubating at room temperature for 1 minute.
- Centrifuge at 6,000 x g for 1 minute to collect the purified genomic DNA.
Final Step :
- For optimal yield, repeat the elution process with a fresh volume of elution buffer if necessary. The purified DNA can now be used for PCR, qPCR, or any downstream applications.