ELISA HiPure Stool DNA

20% OFF list price as part of our FALL Sale! On now until October 18th, 2024! Introduction This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA issuitable for PCR, restriction digestion, and next-generation sequencing. Thereare no organic extractions thus reducing plastic waste and hands-on time to allow mµLtiple samples to be processed in parallel. Details Specifications Features Specifications Main Functions Isolation total DNA from 50-100mg stool samples Applications PCR, Southern Blot, enzyme digestion and NGS, etc. Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifµgation or vacuum) Sample type Stool Sample amount 50-100mg Yield 3-15μg Elution volume ≥30μl Time per run ≤60 minutes Liquid carrying volume per column 750μl Binding yield of column 100μg Principle Stool sample is homogenized and then treated in a specially formµLated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification. Advantages High purity - unique adsorbent can completely remove inhibitory factors High concentration - maximum extraction of total DNA from stool samples High recovery - DNA can be recovered at the level of PG Good repeatability - silica technology can obtain ideal resµLts every time Kit Contents Contents D314102 D314103 Purification Times 50 Preps 250 Preps HiPure DNA Mini Columns II 50 250 2mL Collection Tubes 50 250 2mL Bead Tubes 50 250 Proteinase K 24 mg 120 mg Protease Dissolve Buffer 1.8 mL 10 mL Buffer SPL 40 mL 200 mL Buffer PCI 40 mL 200 mL Buffer AL 20 mL 80 mL Buffer GW1 22 mL 88 mL Buffer GW2 20 mL 2 x 50 mL Buffer AE 15 mL 30 mL Storage and Stability Proteinase K and Buffer PCI shoµLd be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers shoµLd be redissolved before use. Make sure that all buffers are at room temperature when used.