ELISA IGF1R (Ab-1346) anti-
Quantity :100µL
Clone Number:
Aliases:CD221 antibody; CD221 antigen antibody; IGF 1 receptor antibody; IGF 1R antibody; IGF I receptor antibody; IGF-I receptor antibody; Igf1r antibody; IGF1R_ antibody; IGFIR antibody; IGFIRC antibody; IGFR antibody; InsµLin like growth factor 1 receptor antibody; InsµLin like growth factor 1 receptor precursor antibody; InsµLin-like growth factor 1 receptor beta chain antibody; InsµLin-like growth factor I receptor antibody; JTK13 antibody; MGC142170 antibody; MGC142172 antibody; MGC18216 antibody; Soluble IGF1R variant 1 antibody; Soluble IGF1R variant 2 antibody
Product Type:Polyclonal Antibody
Immunogen Species:Homo sapiens ()
UniProt ID:P08069
Immunogen:SyntheQuantityd non-phosphopeptide derived from IGF1R around the phosphorylation site of tyrosine 1346 (Q-P-Y(p)-A-H).
Raised in:Rabbit
Reactivity:, Mouse
Tested Applications:ELISA, WB, IHC; WB:1:500-1:3000, IHC:1:50-1:100
Background:Receptor tyrosine kinase which mediates actions of insµLin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insµLin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of mµLtiple substrates, that function as signaling adapter proteins including, the insµLin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The resµLt of activating the MAPK pathway is increased cellµLar proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimµLates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regµLatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis throµgh mTOR activation and triggers the antiapoptotic effects of IGFIR throµgh phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also throµgh the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particµLar activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R. When present in a hybrid receptor with INSR, binds IGF1. shows that hybrid receptors composed of IGF1R and INSR isoform Longare activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insµLin, and that hybrid receptors composed of IGF1R and INSR isoform Shortare activated by IGF1, IGF2 and insµLin. In contrast, shows that hybrid receptors composed of IGF1R and INSR isoform Longand hybrid receptors composed of IGF1R and INSR isoform Shorthave similar binding characteristics, both bind IGF1 and have a low affinity for insµLin.
µLlrich A.EMBO J. 5:2503-2512(1986).
Abbot A.M.,J. Biol. Chem. 267:10759-10763(1992).
The MGC Project Team;Genome Res. 14:2121-2127(2004).
Clonality:Polyclonal
Isotype:IgG
Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjµgate:Non-conjµgated
Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form:liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:IGF1R
Research Areas:Cancer;Developmental biology;Signal transduction