ELISA AP2M1 anti-

Quantity :50µL Clone Number: Aliases:Adapter-related protein complex 2 mu subunit antibody; Adaptin mu 1 antibody; Adaptin-mu2 antibody; Adaptor protein complex AP 2 subunit mu antibody; Adaptor protein complex AP-2 subunit mu antibody; Adaptor related protein complex 2 mu 1 subunit antibody; AP 2 mu 2 chain antibody; AP-2 complex subunit mu antibody; AP-2 mu chain antibody; Ap2m1 antibody; AP2M1_ antibody; AP50 antibody; CLAPM1 antibody; Clathrin adaptor complex AP2 mu subunit antibody; Clathrin assembly protein complex 2 medium chain antibody; Clathrin associated/assembly/adaptor protein medium 1 antibody; Clathrin coat adaptor protein AP50 antibody; Clathrin coat assembly protein AP50 antibody; Clathrin coat associated protein AP50 antibody; Clathrin coat-associated protein AP50 antibody; HA2 50 kDa subunit antibody; mu2 antibody; Plasma membrane adaptor AP-2 50 kDa protein antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q96CW1 Immunogen:Recombinant AP-2 complex subunit mu protein (136-435AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:20-1:200 Background:Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecµLes. AP-2 may also play a role in maintaining normal post-endocytic trafficking throµgh the ARF6-regµLated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible throµgh a conformational change following phosphorylation of AP-2 mu subunit at \\\'Tyr-156\\\' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:AP2M1 Research Areas:Signal transduction