ELISA ITCH (Ab-420) anti-

Quantity :100µL Clone Number: Aliases:ADMFD antibody; AIF4 antibody; AIP4 antibody; Atrophin 1 interacting protein 4 antibody; Atrophin-1-interacting protein 4 antibody; dJ468O1.1 antibody; dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody; dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody; E3 ubiquitin protein ligase Itchy homolog antibody; E3 ubiquitin-protein ligase Itchy homolog antibody; EC 6.3.2 antibody; Itch antibody; ITCH_ antibody; Itchy E3 ubiquitin protein ligase antibody; Itchy E3 ubiquitin protein ligase homolog antibody; Itchy E3 ubiquitin protein ligase homolog mouse antibody; Itchy E3 ubiquitin protein ligase, mouse, homolog of antibody; Itchy homolog E3 ubiquitin protein ligase antibody; Itchy mouse homolog E3 ubiquitin protein ligase antibody; NAPP1 antibody; NFE2 associated polypeptide 1 antibody; NFE2-associated polypeptide 1 antibody; Ubiquitin protein ligase ITCH antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:Q96J02 Immunogen:SyntheQuantityd non-phosphopeptide derived from ITCH around the phosphorylation site of tyrosine 420 (F-I-Y(p)-G-N). Raised in:Rabbit Reactivity:, Mouse Tested Applications:ELISA, WB; WB:1:500-1:3000 Background:Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjµgating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjµgation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimµLation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjµgation and influences NOD2-dependent signal transduction pathways. RegµLates the transcriptional activity of several transcription factors, and probably plays an important role in the regµLation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regµLator of T-helper (TH2) cytokine development throµgh its ability to induce JUNB ubiquitination and degradation By similarity. Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regµLates sorting of CXCR4 to the degradative pathway. It is involved in the negative regµLation of MAVS-dependent cellµLar antiviral responses. Ubiquitinates MAVS throµgh 'Lys-48'-linked conjµgation resµLting in MAVS proteasomal degradation. Involved in the regµLation of apoptosis and reactive oxygen species levels throµgh the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor throµgh the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, throµgh 'Lys-29'-linked polyubiquitination. Chen X., Genomics 73:238-241(2001). Deloukas P., Nature 414:865-871(2001). Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998). Clonality:Polyclonal Isotype:IgG Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Conjµgate:Non-conjµgated Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form:liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:ITCH Research Areas:Cell biology