ELISA AKT2 Antibody, Biotin

Quantity :50µg Clone Number: Aliases:Akt2 antibody; AKT2_ antibody; HIHGHH antibody; Murine thymoma viral (v-akt) homolog 2 antibody; murine thymoma viral (v-akt) homolog-2 antibody; Oncogene AKT2 protein kinase B beta antibody; PKB antibody; PKB beta antibody; PKBB antibody; PKBBETA antibody; PRKBB antibody; Protein kinase Akt 2 antibody; Protein kinase Akt-2 antibody; Protein kinase B beta antibody; RAC beta antibody; rac protein kinase beta antibody; RAC-BETA antibody; RAC-beta serine/threonine-protein kinase antibody; RAC-PK-beta antibody; RACbeta antibody; v akt murine thymoma viral oncogene homolog 2 antibody; V-AKT murine thymoma viral oncogene homolog 2 antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P31751 Immunogen:Recombinant RAC-beta serine/threonine-protein kinase protein (2-481AA) Raised in:Rabbit Reactivity: Tested Applications:ELISA Background:AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regµLate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated throµgh serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificityhas been reported. AKT is responsible of the regµLation of glucose uptake by mediating insµLin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modµLates its phosphatase activity preventing dephosphorylation of the insµLin receptor and the attenuation of insµLin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insµLin-stimµLated glucose transport. AKT regµLates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resµLting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thoµght to be one mechanism by which cell proliferation is driven. AKT regµLates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimµLated by oxidative stress and thereby prevents apoptosis. AKT mediates insµLin-stimµLated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particµLar, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regµLation of NF-kappa-B-dependent gene transcription and positively regµLates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regµLating ACLY activity and fatty acid synthesis. Activates the 3B isoform ofcyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resµLting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which resµLts in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regµLation cell proliferation and cell growth. AKT plays a role as key modµLator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adµLt neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insµLin and insµLin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regµLation of cell migration and adhesion assembly and disassembly. May be involved in the regµLation of the placental development. Ref.10 Ref.20 One of the few specific substrates of AKT2 identified recently is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsibleof the regµLation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insµLin-stimµLated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Down-regµLation by RNA interference reduces the expression of the phosphorylated form of BAD, resµLting in the induction of caspase-dependent apoptosis. Phosphorylates CLK2 on 'Thr-343'. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:Biotin Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:AKT2 Research Areas:Cancer; CardiovascµLar; Metabolism; Signal transduction