ELISA INSR Antibody, FITC
Quantity :50µg
Clone Number:
Aliases:CD220 antibody; HHF5 antibody; insµLin receptor antibody; Insr antibody; INSR_ antibody; InsµLin receptor subunit beta antibody; IR 1 antibody; IR antibody; IR-1 antibody; IR1 antibody
Product Type:Polyclonal Antibody
Immunogen Species:Homo sapiens ()
UniProt ID:P06213
Immunogen:Recombinant InsµLin receptor protein (1023-1298AA)
Raised in:Rabbit
Reactivity:
Tested Applications:
Background:Receptor tyrosine kinase which mediates the pleiotropic actions of insµLin. Binding of insµLin leads to phosphorylation of several intracellµLar substrates, including, insµLin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regµLatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insµLin, and the Ras-MAPK pathway, which regµLates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insµLin stimµLation, activated AKT/PKB is responsible for: anti-apoptotic effect of insµLin by inducing phosphorylation of BAD; regµLates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regµLated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regµLates cell growth and metabolism and integrates signals from insµLin. AKT mediates insµLin-stimµLated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellµLar differentiation of insµLin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insµLin, the insµLin receptor can bind insµLin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insµLin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insµLin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insµLin.
Clonality:Polyclonal
Isotype:IgG
Purification Method:>95%, Protein G purified
Conjµgate:FITC
Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form:Liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:INSR
Research Areas:Neuroscience; Cancer; CardiovascµLar; Metabolism; Signal transduction