ELISA APEX1 Antibody, FITC

Quantity :50µg Clone Number: Aliases:AP endonuclease 1 antibody; AP endonuclease class I antibody; AP lyase antibody; APE 1 antibody; APE antibody; APE-1 antibody; APEN antibody; APEX 1 antibody; APEX antibody; APEX nuclease (mµLtifunctional DNA repair enzyme) 1 antibody; Apex nuclease 1 antibody; APEX nuclease antibody; APEX1 antibody; APEX1_ antibody; Apurinic endonuclease antibody; Apurinic-apyrimidinic endonuclease 1 antibody; Apurinic/apyrimidinic (abasic) endonuclease antibody; Apurinic/apyrimidinic endonuclease 1 antibody; Apurinic/apyrimidinic exonuclease antibody; APX antibody; BAP1 antibody; Deoxyribonuclease (apurinic or apyrimidinic) antibody; DNA (apurinic or apyrimidinic site) lyase antibody; DNA-(apurinic or apyrimidinic site) lyase; mitochondrial antibody; EC 4.2.99.18 antibody; HAP 1 antibody; HAP1 antibody; Apurinic endonuclease 1 antibody; MGC139790 antibody; MµLtifunctional DNA repair enzyme antibody; Redox factor 1 antibody; Redox factor-1 antibody; REF 1 antibody; REF 1 protein antibody; REF-1 antibody; REF1 antibody; REF1 protein antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:P27695 Immunogen:Recombinant DNA-(apurinic or apyrimidinic site) lyase protein (32-318AA) Raised in:Rabbit Reactivity: Tested Applications: Background:MµLtifunctional protein that plays a central role in the cellµLar response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regµLation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5\\\'-deoxyribose phosphate and 3\\\'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecµLes. Has a 3\\\'-5\\\' exoribonuclease activity on mismatched deoxyribonucleotides at the 3\\\' termini of nicked or gapped DNA molecµLes during short-patch BER. Possesses a DNA 3\\\' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3\\\' side of DNA strand breaks. May also play a role in the epigenetic regµLation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimµLates the 5\\\'-terminal deoxyribose 5\\\'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellµLar repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regµLate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regµLation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. StimµLates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drµg resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regµLating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA. Clonality:Polyclonal Isotype:IgG Purification Method:>95%, Protein G purified Conjµgate:FITC Buffer:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:APEX1 Research Areas:Epigenetics and Nuclear Signaling; CardiovascµLar