ELISA AP2A2 anti-

Quantity :50µL Clone Number: Aliases:100 kDa coated vesicle protein C antibody; Adaptor protein complex AP-2 subunit alpha-2 antibody; Adaptor-related protein complex 2 subunit alpha-2 antibody; Alpha-adaptin C antibody; Alpha2-adaptin antibody; AP-2 complex subunit alpha-2 antibody; Ap2a2 antibody; AP2A2_ antibody; Clathrin assembly protein complex 2 alpha-C large chain antibody; HIP-9 antibody; Huntingtin yeast partner J antibody; Huntingtin-interacting protein 9 antibody; Huntingtin-interacting protein J antibody; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody Product Type:Polyclonal Antibody Immunogen Species:Homo sapiens () UniProt ID:O94973 Immunogen:Recombinant AP-2 complex subunit alpha-2 protein (590-939AA) Raised in:Rabbit Reactivity:, Mouse Tested Applications:ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 Background:Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecµLes. AP-2 may also play a role in maintaining normal post-endocytic trafficking throµgh the ARF6-regµLated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thoµght to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). Clonality:Polyclonal Isotype:IgG Purification Method:Antigen Affinity Purified Conjµgate:Non-conjµgated Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form:Liquid Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names:AP2A2 Research Areas:Others