ELISA Acetyl-NF?B-p65 (Lys314|315) anti-

Quantity:100µL Host:Rabbit Clonality:Polyclonal Isotype:IgG GeneName:RELA GeneID:5970 Uniprot:Q04206 Specificity:Acetyl-NF?B-p65 (Lys314/315) Antibody detects endogenous levels of NF?B-p65 only when acetylated at Lys314/315. Immunogen:A syntheQuantityd peptide derived from NF?B-p65 around the acetylation site of Lys314/315. Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity:,Mouse,Rat Prediction: Application:WB,IHC,ELISA(peptide) Dilution:IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000 Conjµgate:Unconjµgated RRID:AB_2847109 An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide:AF3795-BP Des# Blocking Peptide:Acetyl-NF?B-p65 (Lys314/315) Blocking peptide Blocking Peptide Price:$549 CAD