ELISA Apelin 12 (AP12)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:N/A Abbreviation:AP12 Alternative Names:N/A Application:ELISA Range:Request Information Sensitivity:Request Information Intra-AssayCV:?5.1% Inter-AssayCV:?8.5% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate AP12 in samples. An antibody specific for AP12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAP12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for AP12 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP12 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Apelin, the endogenous peptide ligand of the APJ receptor, is expressed in brain regions implicated in food and water intake. The APJ receptor belongs to a family of 7-transmembrane G-protein coupled receptors.The ligand was named “apelin” after APJ endogenous ligand. Apelin and APJ are widely expressed in homogenates from animal organs in a pattern shared with angiotensinogen and the angiotensin receptor. Apelin is widely distributed in the CNS and periphery, especially in the heart, kidney, lung and mammary glands.present study investigated basal plasma levels of apelin, orexin-A, and leptin in morbid obese patients.Apelin-12 levels also were inversely correlated with levels of nt-proBNP. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).