ELISA Alanyl (membrane) aminopeptidase (ANPEP)

Reactivity: (Homo sapiens) UniProt:P15144 Abbreviation:ANPEP Alternative Names:APN; CD13; GP150; LAP1; P150; PEPN; aminopeptidase M|aminopeptidase N|membrane alanine aminopeptidase|microsomal aminopeptidase|myeloid plasma membrane glycoprotein CD13 Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.57 ng/mL Intra-AssayCV:?3.7% Inter-AssayCV:?6.0% Recovery:0.92 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ANPEP in samples. An antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ANPEP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Alanine aminopeptidase (EC 3.4.11.2) is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is found at high levels in the urine when there are kidney problems.Also called AAP. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubµLar epithelial cells and other cell types is less clear. The large extracellµLar carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).