ELISA Alanine aminotransferase 2 (GPT2)

Reactivity: (Homo sapiens) UniProt:Q8TD30 Abbreviation:GPT2 Alternative Names:ALT2; alanine aminotransferase 2|glutamic pyruvate transaminase 2|glutamic--alanine transaminase|glutamic--pyruvic transaminase|glutamic-pyruvate transaminase 2 Application:ELISA Range:12.5-800 pg/mL Sensitivity:5.4 pg/mL Intra-AssayCV:?4.8% Inter-AssayCV:?9.2% Recovery:0.95 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate GPT2 in samples. An antibody specific for GPT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for GPT2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Angiopoietin-2 (Ang-2) is a secreted glycoprotein that plays a complex role in vascµLar development. Ang-2 contains an N-terminal coiled-coildomain, 58 - 254 amino acids (aa) and a C-terminal fibrinogen-like domain, 281 - 467 aa, that is also found in other proteins including fibrinogen, tenascin, and ficolin. Located at the N-terminus are stretches of hydrophobic residues typical of secretory signal sequences. While Ang-2 is widely expressed in the mouse embryo, it is restricted postnatally to pro-angiogenic regions including the placenta, ovaries, and uterus. Ang-2 has been implicated in cancer development due to its role in angiogenesis.Ang-2-mediated destabilization of mature vessel structures accompanied by the presence of VEGF, may contribute to the angiogenesis associated with tumor tissues. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).