ELISA Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L)
Reactivity: (Homo sapiens)
UniProt:Q8TEK3
Abbreviation:DOT1L
Alternative Names:DKFZp586P1823; DOT1; KIAA1814; KMT4; DOT1-like; histone H3 methyltransferase|histone methyltransferase DOT1L
Application:ELISA
Range:0.156-10 ng/mL
Sensitivity:0.059 ng/mL
Intra-AssayCV:?5.7%
Inter-AssayCV:?8.3%
Recovery:0.97
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate DOT1L in samples. An antibody specific for DOT1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOT1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for DOT1L is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOT1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DOT1L is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. DOT1L is a nucleosomal histone-3 (H3)-specific methyltransferase that requires the SAM and sequences between amino acids 351 and 472 for activity. Overexpression of DOT1L in embryonic kidney cells significantly increased methylation of lys79 of H3, but had no effect on lys4 or lys9. Methylation of lys79, attributed to DOT1L, was regµLated during the cell cycle in synchronized HeLa cells. The deduced 1,537-amino acid protein has a calcµLated molecµLar mass of 165 kD and contains a SAM (sterile alpha motif) domain for protein-protein interaction. DOT1L does not, however, have the SET domain found in most histone lysine methyltransferases.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).