ELISA Horse Estrogen (E)
Reactivity:Horse (Equus caballus; Equine)
UniProt:N/A
Abbreviation:E
Alternative Names:N/A
Application:ELISA
Range:18.75-13.125-200 pg/mL
Sensitivity:10.2 pg/mL
Intra-AssayCV:?5.1%
Inter-AssayCV:?9.2%
Recovery:1.06
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate E in samples. An antibody specific for E has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for E is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of E bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Estrogen (American English) or oestrogen (British English) is the primary female sex hormone. It is responsible for the development and regµLation of the female reproductive system and secondary sex characteristics. Estrogen may also refer to any substance, natural or synthetic, that mimics the effects of the natural hormone. The steroid 17?-estradiol is the most potent and prevalent endogenousestrogen, althoµgh several metabolites of estradiol also have estrogenic hormonal activity. Synthetic estrogens are used as part of some oral contraceptives, in estrogen replacement therapy forpostmenopausal women, and in hormone replacement therapy for trans women.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).