ELISA Interleukin 9 (IL-9)
Reactivity: (Homo sapiens)
UniProt:P15248
Abbreviation:IL-9
Alternative Names:HP40; IL9; P40; T-cell growth factor p40|homolog of mouse T cell and mast cell growth factor 40|p40 T-cell and mast cell growth factor|p40 cytokine
Application:ELISA
Range:15.6-1000 pg/mL
Sensitivity:7.8 pg/mL
Intra-AssayCV:?7.4%
Inter-AssayCV:?10.6%
Recovery:1.01
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate IL-9 in samples. An antibody specific for IL-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for IL-9 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).