ELISA Kinesin-like protein KIF14 (KIF14)

Reactivity: (Homo sapiens) UniProt:Q15058 Abbreviation:KIF14 Alternative Names:KIAA0042; MGC142302; Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.126 ng/mL Intra-AssayCV:?3.9% Inter-AssayCV:?7.6% Recovery:0.89 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KIF14 in samples. An antibody specific for KIF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KIF14 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF14 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:KIF14 is a member of the kinesin superfamily of microtubµLe-associated motors that play important roles in intracellµLar transport and cell division KIF14 protein contains an internal motor domain with a conserved catalytic core, an N-type conserved neck region, a forkhead-associated (FHA) domain, and 4 coiled-coil domains. Immunofluorescence microscopy localized KIF14 throµghout the cell cycle in 293T cells and HeLa cells. During interphase, KIF14 localized to the cytoplasm and was redistributed to the nucleus upon entry into mitosis. From prophase to metaphase, KIF14 localized at developing spindle poles and associated microtubµLes, and during anaphase, KIF14 accumµLated at the spindle midzone, where it concentrated during telophase. KIF14 localized to the contractile ring in cells ready to undergo abscission. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).