ELISA Kinesin-like protein KIF1A (KIF1A)

Reactivity: (Homo sapiens) UniProt:Q12756 Abbreviation:KIF1A Alternative Names:ATSV; C2orf20; DKFZp686I2094; FLJ30229; HUNC-104; MGC133285; MGC133286; UNC104; axonal transport of synaptic vesicles|kinesin; heavy chain; member 1A; homolog of mouse|tmp_locus_27 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.051 ng/mL Intra-AssayCV:?3.5% Inter-AssayCV:?6.5% Recovery:0.94 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KIF1A in samples. An antibody specific for KIF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KIF1A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:KIF1A is a member of the kinesin family. This protein is highly similar to mouse heavy chain kinesin member 1A protein which is an anterograde motor protein that transports membranous organelles along axonal microtubµLes. It is thoµght that this protein may play a critical role in the development of axonal neuropathies resµLting from impaired axonal transport. There are mµLtiple polyadenylation sites found in this gene. This switch-like transition for movement coµLd be shifted to lower PtdIns(4,5)P2 concentrations by the addition of cholesterol/sphingomyelin or GM1 ganglioside/cholera toxin, conditions that produced raft-like behavior of Unc104 bound to lipid bilayers. The authors concluded that clustering of Unc104 in PtdIns(4,5)P2-containing rafts provides a trigger for membrane transport. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).