ELISA Kinesin heavy chain isoform 5A (KIF5A)

Reactivity: (Homo sapiens) UniProt:Q12840 Abbreviation:KIF5A Alternative Names:D12S1889; MY050; NKHC; SPG10; KIF5A variant protein|kinesin; heavy chain; neuron-specific Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.112 ng/mL Intra-AssayCV:?4.6% Inter-AssayCV:?7.6% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KIF5A in samples. An antibody specific for KIF5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KIF5A is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF5A bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The predicted 1,032-amino acid protein has the characteristic features of a KHC, as well as a unique C-terminal stretch of 69 amino acids. The amino acid sequence of NKHC is 65% and 54% identical to the amino acid sequences of KNS1 and the Drosophila KHC. Northern blot analysis of rat tissues detected Nkhc expression only in brain, where mµLtiple transcripts were found. Immunoblot analysis of rat tissue extracts using antibodies specific for NKHC detected a doublet of approximately 120 and 133 kD only in brain and sciatic nerve tissue. By indirect immunofluorescence, the authors showed that NKHC is distributed throµghout the central nervous system but is highly enriched in subsets of neurons. Within cµLtured hippocampal neurons, NKHC is concentrated in the cell body, particµLarly in the perinuclear region. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).