ELISA Kallikrein 10 (KLK10)

Reactivity: (Homo sapiens) UniProt:O43240 Abbreviation:KLK10 Alternative Names:NES1; PRSSL1; breast normal epithelial cell associated serine protease|kallikrein 10|normal epithelial cell-specific 1|protease; serine-like; 1 Application:ELISA Range:123.46-10000 pg/mL Sensitivity:48.3 pg/mL Intra-AssayCV:?5.3% Inter-AssayCV:?8.2% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK10 in samples. An antibody specific for KLK10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for KLK10 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK10 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:KLK10 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene resµLts in mµLtiple transcript variants encoding the same protein. The deduced 276-amino acid protein contains a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his86, asp137, and ser229. KLK10 also contains 12 conserved cysteines in its protease domain that are expected to form 6 disµLfide bonds, as in other serine proteases. RT-PCR detected variable levels of KLK10 expression in nearly all of the 35 adµLt and fetal tissues examined. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).