ELISA Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2)

Reactivity: (Homo sapiens) UniProt:Q6ISS4 Abbreviation:LAIR2 Alternative Names:XXbac-BCX535A19.6; CD306; MGC71634; leukocyte-associated Ig-like receptor 2|leukocyte-associated Ig-like receptor-2 Application:ELISA Range:15.6-1000 pg/mL Sensitivity:6.4 pg/mL Intra-AssayCV:?5.3% Inter-AssayCV:?10.3% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LAIR2 in samples. An antibody specific for LAIR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAIR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LAIR2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAIR2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:LAIR 2 is a member of the immunoglobµLin superfamily. It was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. This gene maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobµLin superfamily, including LAIR1. The function of this protein is unknown, althoµgh it is thoµght to be secreted and may help modµLate mucosal tolerance. Two transcript variants encoding different isoforms have been found for this gene.By screening a T-cell cDNA library,Meyaard et al. isolated a LAIR2 cDNA. The predicted LAIR2 protein contains 1 immunoglobµLin-like domain, which is 84% homologous to that of LAIR1; it lacks both a transmembrane and a cytoplasmic domain, and therefore the authors sµggested that it is secreted. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).