ELISA Laminin subunit beta-2 (LAMB2)

Reactivity: (Homo sapiens) UniProt:P55268 Abbreviation:LAMB2 Alternative Names:LAMS; OTTHUMP00000210663|laminin S|laminin; beta 2 Application:ELISA Range:0.78-50 ng/mL Sensitivity:0.31 ng/mL Intra-AssayCV:?3.7% Inter-AssayCV:?7.2% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMB2 in samples. An antibody specific for LAMB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LAMB2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMB2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Laminin subunit beta-2 is the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscµLar junctions, kidney glomerµLus and vascµLar smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscµLar junctions and impairment of glomerµLar filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was sµggested that inefficient splicing of this first intron, which does not change the protein sequence, resµLts in a greater abundance of the un. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).