ELISA Lipocalin-1 (LCN1)

Reactivity: (Homo sapiens) UniProt:P31025 Abbreviation:LCN1 Alternative Names:MGC71975; PMFA; TP; VEGP; OTTHUMP00000022539|Von Ebner gland protein|lipocalin 1|lipocalin 1 (protein migrating faster than albumin; tear prealbumin)|lipocalin 1-like 2|tear lipocalin Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.62 ng/mL Intra-AssayCV:?5.6% Inter-AssayCV:?9.4% Recovery:1.04 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LCN1 in samples. An antibody specific for LCN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LCN1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCN1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Lipocalin-1 belongs to the lipocalin family. Lipocalins are a group of extracellµLar proteins that are able to bind lipophiles by enclosure within their structures to minimize solvent contact. This protein may bind hydrophobic ligands and inhibit cysteine proteinases. It may also play a role in taste reception. Based on the known 3-dimensional structure of 5 members of the lipocalin family, i.e., retinol binding protein , beta-lactoglobµLin, bilin binding protein, mouse major urinary protein, and rat urinary alpha-2-globµLin, the general architecture appears to be highly appropriate for binding a variety of hydrophobic ligands. On the basis of highly conserved amino acid sequences and of a Quantity around 18 to 20 kD, about 20 proteins have been designated as lipocalins. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).