ELISA L-lactate dehydrogenase B chain (LDHB)

Reactivity: (Homo sapiens) UniProt:P07195 Abbreviation:LDHB Alternative Names:LDH-H; TRG-5; L-lactate dehydrogenase B|OTTHUMP00000165229 Application:ELISA Range:23.44-1500 pg/mL Sensitivity:5.8 mU/mL Intra-AssayCV:?5.1% Inter-AssayCV:?10.2% Recovery:1.03 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LDHB in samples. An antibody specific for LDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LDHB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:LDHB and peptidase B are linked (Santachiara et al., 1970) and both loci are on chromosome 12 (Chen et al., 1973). Kitamura et al. (1971) reported the first case of a complete deficiency of lactate dehydrogenase subunit H(B) in serum, saliva and erythrocytes of a 64-year-old male with mild diabetes. Study made on family members revealed low LDH activity in their serum also linked with decreased relative activity of the H4(B4) fraction. Based on the comparison of the calcµLated ratio of H to M subunits in normal and affected family members, it was hypotheQuantityd that the proband is homozygous while the abnormal family members are heterozygous, assuming a single gene is involved. Red cell metabolism in the proband was studied by Miwa et al. (1971); neither reticµLocytosis nor hemolytic anemia was present. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).