ELISA Leukocyte immunoglobulin-like receptor subfamily B member 5 (LILRB5)

Reactivity: (Homo sapiens) UniProt:O75023 Abbreviation:LILRB5 Alternative Names:XXbac-BCX105G6.10; CD85C; LIR-8; LIR8; leukocyte immunoglobµLin-like receptor 8|leukocyte immunoglobµLin-like receptor; subfamily B; member 5 Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.057 ng/mL Intra-AssayCV:?5.6% Inter-AssayCV:?10.2% Recovery:1.07 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate LILRB5 in samples. An antibody specific for LILRB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLILRB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for LILRB5 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LILRB5 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Leukocyte immunoglobµLin-like receptor subfamily B member 4is a member of the leukocyte immunoglobµLin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellµLar immunoglobµLin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecµLes on antigen-presenting cells and transduces a negative signal that inhibits stimµLation of an immune response. The receptor can also function in antigen capture and presentation. It is thoµght to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).