ELISA Goat Myoglobin (MB)

Reactivity:Goat (Capra hircus; Caprine) UniProt:B7U9B5 Abbreviation:MB Alternative Names:MGC13548; PVALB; OTTHUMP00000197922 Application:ELISA Range:31.25-2000 pg/mL Sensitivity:7.82 pg/mL Intra-AssayCV:?4.3% Inter-AssayCV:?10.5% Recovery:0.99 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate MB in samples. An antibody specific for MB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for MB is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MB bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Myoglobin is a single-chain globµLar protein of 153 amino acids, containing a heme prosthetic group in the center around which the remaining apoprotein folds. It has eight alpha helices and a hydrophobic core. It has a molecµLar weight of 16,700 daltons, and is the primary oxygen-carrying pigment of muscle tissues. Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperativity is a property of mµLtimeric/oligomeric proteins only. Instead, the binding of oxygen by myoglobin is unaffected by the oxygen pressure in the surrounding tissue. Myoglobin is often cited as having an "instant binding tenacity" to oxygen given its hyperbolic oxygen dissociation curve. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).