ELISA ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13)

Reactivity: (Homo sapiens) UniProt:N/A Abbreviation:ADAMTS13 Alternative Names:C9orf8; DKFZp434C2322; FLJ42993; MGC118899; MGC118900; TTP; VWFCP; vWF-CP; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 13|vWF-cleaving protease|von Wil Application:ELISA Range:31.25-2000 pg/mL Sensitivity:15.5 pg/mL Intra-AssayCV:?5.8% Inter-AssayCV:?8.6% Recovery:1.06 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ADAMTS13 in samples. An antibody specific for ADAMTS13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ADAMTS13 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS13 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13)—also known as von Willebrand factor-cleaving protease (VWFCP)—is a zinc-containing metalloprotease enzyme that cleaves von Willebrand factor (vWf), a large protein involved in blood clotting. It is secreted in blood and degrades large vWf mµLtimers, decreasing their activity.Genomically, ADAMTS13 shares many properties with the 19 member ADAMTS family, all of which are characterised by a protease domain (the part that performs the protein hydrolysis), an adjacent disintegrin domain and one or more thrombospondin domains. ADAMTS13 in fact has eight thrombospondin domains. It has no hydrophobic transmembrane domain, and hence it not anchored in the cell membrane. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).