ELISA Apoptosis-associated speck-like protein containing a CARD (PYCARD)

Reactivity: (Homo sapiens) UniProt:Q9µLZ3 Abbreviation:PYCARD Alternative Names:ASC; CARD5; MGC10332; TMS; TMS-1; TMS1; apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain protein 5|target of methylation-induced silencing-1 Application:ELISA Range:15.6-1000 pg/mL Sensitivity:3.9 pg/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.4% Recovery:0.87 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate PYCARD in samples. An antibody specific for PYCARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYCARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for PYCARD is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCARD bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:PYCARD encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery.The deduced 195-amino acid protein contains an N-terminal pyrin-like domain (PYD) and an 87-residue C-terminal CARD. Fluorescence microscopy demonstrated a ring-like expression in some transfected cells. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).