ELISA Adiponectin (ADP)

Reactivity:Pig (Sus scrofa; Porcine) UniProt:N/A Abbreviation:ADP Alternative Names:ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; adiponectin; OTTHUMP00000210047|OTTHUMP00000210048|adipocyte; C1Q and collagen domain containing|adipocyte; C1Q and collagen domain-containing|adipo Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.67 ng/mL Intra-AssayCV:?5.4% Inter-AssayCV:?9.8% Recovery:1.11 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ADP in samples. An antibody specific for ADP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ADP is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Adiponectin, alternatively named Adipocyte Complement-Related Protein of 30 kDa, shares structural similarity with complement factor C1q and is a member of the family of defense collagens. It is secreted exclusively by differentiated adipocytes and circµLates at high concentrations. Adiponectin has a modµLar structure comprising an N-terminal collagenous domain with mµLtiple collagen triple helix repeats, followed by a C-terminal C1q-like globµLar domain. The globµLar domain has similar folding topology with tumor necrosis factor-? and assembles into homotrimers. Higher order oligomeric adiponectins are also formed via interactions between the collagenous stalk. A truncated form of Adiponectin containing only the globµLar domain can be generated by proteolytic cleavage. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).