ELISA Actin, alpha skeletal muscle (ACTA1)

Reactivity: (Homo sapiens) UniProt:P68133 Abbreviation:ACTA1 Alternative Names:RP5-1068B5.2; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; alpha skeletal muscle actin Application:ELISA Range:1.56-100 ng/mL Sensitivity:0.57 ng/mL Intra-AssayCV:?4.9% Inter-AssayCV:?8.2% Recovery:0.9 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ACTA1 in samples. An antibody specific for ACTA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ACTA1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Skeletal alpha actin expression is induced by stimµLi and conditions known to cause muscle formation. Such conditions resµLt in fusion of committed cells (satellite cells) into myotubes, to form muscle fibers. Skeletal actin itself, when expressed, causes expression of several other "myogenic genes", which are essential to muscle formation. One key transcription factor that activates skeletal actin gene expression is Serum Response Factor ("SRF"), a protein that binds to specific sites on the promoter DNA of the actin gene. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).