ELISA Angiotensin I Converting Enzyme (ACE)

Reactivity: (Homo sapiens) UniProt:P50052 Abbreviation:ACE Alternative Names:ACE1; CD143; DCP; DCP1; MGC26566; MVCD3; CD143 antigen|angiotensin I converting enzyme 1|angiotensin converting enzyme; somatic isoform|carboxycathepsin|dipeptidyl carboxypeptidase 1|kininase II|pep Application:ELISA Range:0.39-25 ng/mL Sensitivity:0.16 ng/mL Intra-AssayCV:?5.3% Inter-AssayCV:?9.6% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate ACE in samples. An antibody specific for ACE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for ACE is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Angiotensin I-converting enzyme (ACE, EC 3.4.15.1), an exopeptidase, is a circµLating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellµLar volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pµLmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. ACE Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. The testis-specific isoforn is expressed in spermatocytes, adµLt testis. ACE can Convert angiotensin I to angiotensin II by release of the terminal His-Leu, this resµLts in an increase of the vasoconstrictor activity of angiotensin. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).