ELISA GTP:AMP phosphotransferase, mitochondrial (AK3)

Reactivity: (Homo sapiens) UniProt:Q9UIJ7 Abbreviation:AK3 Alternative Names:RP11-6J24.4; AK3L1; AK6; AKL3L; AKL3L1; FIX; adenylate kinase 3 alpha like|adenylate kinase 3 like 1|adenylate kinase 6; adenylate kinase 3 like 1 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.146 ng/mL Intra-AssayCV:?6.7% Inter-AssayCV:?10.2% Recovery:1 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate AK3 in samples. An antibody specific for AK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for AK3 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AK3 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:The deduced 227-amino acid protein has a calcµLated molecµLar mass of 25.6 kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1). It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecµLar mass of 28 kD in all tissues examined. SubcellµLar fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction. embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).