ELISA Interferon-inducible protein AIM2 (AIM2)

Reactivity: (Homo sapiens) UniProt:O14862 Abbreviation:AIM2 Alternative Names:PYHIN4; Application:ELISA Range:0.156-10 ng/mL Sensitivity:0.063 ng/mL Intra-AssayCV:?5.8% Inter-AssayCV:?8.4% Recovery:1.02 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate AIM2 in samples. An antibody specific for AIM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAIM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for AIM2 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIM2 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:AIM2 plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. AIM2 was expressed as a 39-kD protein following in vitro transcription/translation in rabbit reticµLocyte lysate or transfection in a murine epithelial cell line. Cell fractionation showed that AIM2 localized primarily to the cytoplasm of transfected mouse cells. Chromosome alterations in malignant melanoma most frequently include translocations and deletions on chromosome 1 or 6 AIM2 encodes a deduced 344-amino acid protein that contains a conserved sequence domain of approximately 200 amino acids shared with interferon-inducible genes as well as a tyrosine kinase phosphorylation site. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).