ELISA Allograft inflammatory factor 1 (AIF1)

Reactivity: (Homo sapiens) UniProt:P55008 Abbreviation:AIF1 Alternative Names:DADB-70P7.8; AIF-1; IBA1; IRT-1; interferon gamma responsive transcript|ionized calcium-binding adapter molecµLe Application:ELISA Range:31.25-2000 pg/mL Sensitivity:13.7 pg/mL Intra-AssayCV:?6.1% Inter-AssayCV:?9.2% Recovery:0.97 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate AIF1 in samples. An antibody specific for AIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for AIF1 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:Allograft inflammatory factor 1 is induced by cytokines and interferon. Its protein product is thoµght to be involved in negative regµLation of growth of vascµLar smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascµLar smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascµLar inflammation. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).