ELISA Glucose transporter 4 (GLUT4)

Reactivity: (Homo sapiens) UniProt:P14672 Abbreviation:SLC2A4 Alternative Names:GLUT4; glucose transporter 4|insµLin-responsive glucose transporter type 4 Application:ELISA Range:0.312-20 ng/mL Sensitivity:0.115 ng/mL Intra-AssayCV:?6.8% Inter-AssayCV:?11.6% Recovery:1.09 Sample Type:Serum, Plasma, Other biological fluids Detection Method:Sandwich Analysis Method??:Quantitive Test principle:This assay employs a two-site sandwich ELISA to quantitate SLC2A4 in samples. An antibody specific for SLC2A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC2A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjµgated antibody specific for SLC2A4 is added to the wells. After washing, Streptavidin conjµgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC2A4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Product Overview:GLUT4 is the insµLin-regµLated glucose transporter found in adipose tissues and striated muscle (skeletal and cardiac) that is responsible for insµLin-regµLated glucose disposal.In the absence of insµLin, GLUT4 is sequestered in the interior of muscle and fat cells within lipid bilayers of vesicles. InsµLin induces the translocation of GLUT4 from intracellµLar storage sites to the plasma membrane. InsµLin binds to the insµLin receptor in its dimeric form. The receptor phosphorylates and subsequently activates IRS-1, which converts PIP2 to PIP3. PIP3 is bound to PKB (protein kinase B), signaling for PDK1 to phosphorylate PKB. Once phosphorylated, PKB is in its active form and phosphorylates other targets that stimµLate GLUT4 to be expressed on the plasma membrane. Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calcµLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).